Kolmisilmä or Kolmisilmän koipi cannot die by any means for a pacifist run, including through methods that would normally count as a trick kill (such as Impact damage). Other bosses might not invalidate the run if killed with non-player damage; e.g. killing Sauvojen tuntija with the "Bridge Boss Cheese" method does not count as a player kill.
Kicking will count as player kills; be careful when trying to kick physics objects into enemies when they are too close.
Glueballs from the Glue Hiisi can be killed and count as player kills, ruining pacifist.
Also killing things such as Flasks/Hollow Eggs/Bombs/Deercoy etc. will count as you killing an entity. Exception being if the spell is pacifist.
Even if using a pacifist safe wand killing Houre crystal will be considered you killing them.
A new mechanic with teleporting at high speeds builds up a mini explosion. This is NOT pacifist safe.
A good way to tell if you have failed this run is when picking up a perk the altar will bleed (the exception to this is if there is a wand from the pacifist chest this will make the altar bleed even if you haven't killed anything)
Spells
Safe
Long Distance Cast + Explosive Projectile + Summon Hollow Egg
Any projectile when Cessation is cast before it deals damage. If Cessation is cast before the release of a payload, payload kills will also be trick kills (except for some special cases such as Eldritch Portal).
Explosive Detonator ending any other projectile that explodes. If a projectile is ended before its lifetime ends, the explosion will not carry info about the caster. Nothing is safe to the caster, but is a trick kill.
Explosives with a physics body (such as Bomb/Summon Explosive Box/Summon Large Explosive Box) will count as pacifist trick kills if they explode from dying to non-player-associated damage, from random chance on being hit, from having their physics body destroyed, or from alternative component lifetime caps expiring (but will count as player kills if they explode from their "normal" (ProjectileComponent) lifetime expiring, and while explosions from dying to player-associated damage are trick kills, the player will still be credited with a kill of the explosive itself)
Nonpacifist payloads inside a Hollow Egg will generate player kills unless using the caster unknown trick. (Explosive Projectile on hollow eggs also generates pacifist trick kills via its explosion.) Hollow eggs left behind can also be "killed" and be credited against the player if they were not killed via a pacifist method.
Some modifiers can be used safely as long as the projectile they are modifying does not kill the enemy. Projectile damage can be reduced with Light Shot or Piercing Shot, among others. Every Modifier though dangerous can be made in to pacifist safe land mines with Intense Concentrated Light interactions.
Laser Bounce/Plasma Beam Bounce (Emitting projectile is not safe (ie. if an enemy jumps into the projectile itself it will count as a kill) but the beam is safe)
Plasma Beam Orbit/Nuke Orbit (Emitting projectile is not safe (ie. if an enemy jumps into the projectile itself it will count as a kill) but the explosion and beam are safe)
Emerald Tablets act as both a 25 damage projectile and a physics body, and will count as player kills if the projectile kills the target. This makes them very unsafe to use on enemies, although slightly safer to use on enemies with more than 25 HP. However, if you drag an item out of your inventory instead of equipping it and throwing it, you can "throw" an item without counting as a player kill. This also works with other unsafe items listed below.
Projectiles from Broken Wand do not count as a player kill, but hitting enemies with the Broken Wand itself will.
Elemental stones like the Ukkoskivi, while safe to use in water for trick kills, should not be thrown at enemies, as kills from hitting enemies with them will count as player kills.
Projectiles transformed by Runestones will retain ownership and will still count as your kills if lethal
Flasks and Eggs, if broken via a non-pacifist spell, will count as a player kill
(but breaking via throwing or launching is safe)
Exploit (patched)
This content includes possible spoilers or secrets. Tread carefully.
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The easiest method by far to achieve a pacifist run was to use the polymorphine exploit, as detailed in the below video. This has been patched and therefore only works on Noita versions released before March 11, 2023.
Impact damage). Other bosses might not invalidate the run if killed with non-player damage; e.g. killing Sauvojen tuntija with the "Bridge Boss Cheese" method does not count as a player kill.
Projectile damage can be reduced with Light Shot or Piercing Shot, among others. Every Modifier though dangerous can be made in to pacifist safe land mines with Intense Concentrated Light interactions.